THE ISOLATION OF CILIARY BASAL BODIES (KINETOSOMES) FROM TETRAHYMENA PYRIFORMIS
نویسندگان
چکیده
منابع مشابه
The Isolation of Ciliary Basal Bodies (kinetosomes) from Tetrahymena Pyriformis
S e a m a n (7, 8) has devised a m e t h o d for the isolation of ciliary basal bodies (kinetosomes) from Tetrahymena pyriformis a n d has presented d a t a on the c h e m i c a l properties of his fraction. However, only light m i c r o s c o p y was e m p l o y e d as a m e a n s of identification of the kinetosomes. I n the experi-m e n t s described here the m a t e r i a l o b t a i n e d ...
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Pellicular fragments were isolated from ethanol-fixed cells of the holotrichous ciliate Tetrahymena pyriformis by the action of digitonin. The isolated pellicles were further fragmented and the basal bodies of the cilia isolated from them by three methods. The preparations, examined in the electron microscope as embedded sections or negatively stained samples, consisted mainly of somewhat defor...
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The numbers of kinetosomes in ciliary rows on the ventral surface of cells of Tetrahymena pyriformis, syngen 1, are greater by about 10% in young heterozygotes established by crossing two inbred strains than in either homozygote. The difference, apparent at 20 fissions post-fertilization, is essentially gone by 100 fissions. This observation is consistent with a generalized "allelic exclusion" ...
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A new procedure for mass isolation of pure pellicles containing intact basal bodies of Tetrahymena pyriformis is reported. The success of the procedure depends on the elimination of the sticky mucocyst contents before fractionation of the cells, which is induced by Alcian Blue 8GS. Under appropriate ionic conditions greater than 95% of the cells are able to form a capsule by simultaneous extrus...
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We have examined thin sections and replicas of freeze-fractured cilia of Tetrahymena pyriformis. The ciliary necklace located at the base of all freeze-fractured oral and somatic cilia has been studied in thin sections. Since electron-dense linkers have been found to connect both microtubule doublets and triplets to the ciliary membrane at the level of the necklace, the linkers and the associat...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1965
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.24.1.154